一种测定黄曲霉毒素的方法

Method for determining aflatoxin

Abstract

The invention discloses a method for determining aflatoxin. The method is characterized by comprising the following steps: (1) preparing a solution 1 for later use; (2) preparing a solution 2 for later use; (3) preparing R1 modified by HRP (horseradish peroxidase); (4) preparing R2 modified by HRP; and (5) detecting aflatoxin. Red rhodamine B can neither cause concentration quenching, nor cause insufficient light intensity. By adding mica powder, the luminescence lifetime of an oxalate luminescence system is significantly prolonged. Ruthenium terpyridine and chemiluminescence have the advantages of high detection sensitivity, wide linear range and the like, and aflatoxin can be detected more effectively. The method disclosed by the invention has the excellent properties of lower toxicity, higher flash point and lower vapor pressure. By detecting the content of aflatoxin in liquid milk and using a standard addition recovery method, the recovery yield of 98.4%-100.4% is obtained, indicating that the method has good accuracy in determination of aflatoxin.
本发明公开了一种测定黄曲霉毒素的方法,其特征在于,所述方法包括以下步骤:(1)待用液1的制备(2)待用液2的制备(3)制备HRP(辣根过氧化物酶)修饰的R1(4)制备HRP修饰的R2(5)黄曲霉毒素的检测。红色罗丹明B既不会引起浓度淬灭,也不会导致光强不足。云母粉末的加入使草酸酯发光体系的发光寿命显著延长。三联吡啶钌和化学发光具有检测灵敏度高、线性范围宽等优点,能够更加有效的检测黄曲霉毒素。本发明具有更低的毒性、更高的闪点和更低的蒸气压这些优良特性。通过检测液态奶中黄曲霉毒素的含量,并利用加标回收法得到回收率在98.4%-100.4%,表明此方法在测定黄曲霉毒素时具有良好的准确度。

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Cited By (1)

    Publication numberPublication dateAssigneeTitle
    CN-104964969-AOctober 07, 2015广东省生态环境与土壤研究所Label-free visualization detection method and label-free visualization detection kit of aflatoxin B1