Preparation method of monensin premix

莫能菌素预混剂的制备方法

Abstract

The invention discloses a preparation method of a monensin premix. The preparation method comprises the following specific steps: preparing cinnamon streptomyce strain; seed flask culture and seed tank culture are performed; when the age of the seed is 20-24h, the pH of a culture solution is 6.6-7.1, the biomass is above 10%, and microscopic examination shows that mycelia are thick and reticular, are stretched and darkly stained and are free of foreign bacteria, performing fermentation culture is performed; when the residual oil amount is below 0.7%, the biomass is above 40%, the mycelium concentration is 40-50%, and the monensin titer is not lower than 40000U/mL, fermentation is finished; monensin is extracted by a one-step spray drying method, wherein the prepared finished monensin product has the monensin content of above 20% and the grain rate of above 75%. According to the preparation method, the fermentation process is simple, so that the fermentation unit can be effectively increased and the production cost can be reduced; usages of an animal-derived nitrogen source and a chemical organic solvent are avoided.
本发明公开了一种莫能菌素预混剂的制备方法,具体为将肉桂地链霉菌菌种制备后先进行种子摇瓶培养和种子罐培养,当培养至种子龄为20~24h,培养液pH值为6.6~7.1,生物量为10%以上,镜检结果显示菌丝粗壮呈网状、舒展、染色深、无杂菌时,转为发酵培养。待残油量为0.7%以下,生物量在40%以上,菌丝浓度为40~50%,莫能菌素效价不低于40000u/mL时,发酵结束。最后采用一步喷雾干燥法进行莫能菌素的提取,所制得的莫能菌素成品含量为20%以上,颗粒率达75%以上。另外,该方法发酵工艺简单,能有效提高发酵单位,降低生产成本,同时避免了动物源性氮源以及化学有机溶剂的使用。

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Patent Citations (2)

    Publication numberPublication dateAssigneeTitle
    CN-102899377-AJanuary 30, 2013金河生物科技股份有限公司Preparation method of chlortetracycline premix
    CN-103937848-AJuly 23, 2014宁夏泰瑞制药股份有限公司一种肉桂地链霉菌发酵生产莫能菌素的新型培养基和培养方法

NO-Patent Citations (1)

    Title
    CHAOXUAN LI ET AL.: "Crotonyl-coenzyme A reductase provides methylmalonyl-CoA precursors for monensin biosynthesis by Streptomyces cinnamonensis in an oil-based extended fermentation", 《MICROBIOLOGY》

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