The invention discloses a sectional type high-efficiency column-mounting column chromatography combination device capable of adjusting a column temperature and freely splitting. The column chromatography combination device is composed of a sectional type chromatographic tube, a sectional type temperature control tube, a fixed frame, a pump type circulating water bath kettle or a low-temperature cooling liquid circulating pump. Free customization of the chromatographic tube column volume is achieved through dismounting and additionally mounting chromatographic tube column units, and sectional column mounting, close-distance sample addition and dismountable cleaning can be achieved. Through additionally mounting the sectional type temperature control tube and connecting the pump type circulating water bath kettle or the low-temperature cooling liquid circulating pump, the column temperature can be adjusted. The column chromatography combination device has the beneficial effects that the column chromatography combination device is capable of sectional dismounting, mounting and combination, and control the column temperature, can freely customize the column volume, adjusts the column temperature, is better in separation effect and higher in separation efficiency, can replace cataclastic parts, and makes a column chromatography experiment operation process more simple, accurate and efficient.